It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species, we recommend using FabRICATOR Z.įabRICATOR is cloned from Streptococcus pyogenes and expressed in E. The best activity is obtained at 37☌, but digestion can also be performed at room temperature using a prolonged reaction time.įabRICATOR digests all subclasses of human, monkey, rabbit and sheep IgG. Since FabRICATOR digests IgG under physiological reaction conditions, the immunoreactivity is preserved. The IgG is digested within 30 minutes, and there is no risk of overdigestion if the incubation time is prolonged. Anti-FabRICATOR - a goat polyclonal antibody directed towards the FabRICATOR enzymeįabRICATOR (IdeS) is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab')2 and Fc/2 fragments.FabRICATOR MagIC - immobilized enzyme on magnetic agarose beads for fast parallel digestion of multiple antibodiesĪlso, a method to detect the FabRICATOR Enzyme is available:.FabRICATOR-HPLC - immobilized enzyme in an HPLC column for fast online digestion of monoclonal antibodies.FabRICATOR Validation kit - consists of three batches of the enzyme to simplify method validation.FabRICATOR LE - lyophilized enzyme with low levels of endotoxins.FragIT kit - immobilized enzyme and affinity purification resin for generation and purification of F(ab')2 and Fc/2 fragments from IgG.This article has been viewed 153,244 times.FabRICATOR (IdeS) is a cysteine protease that digests antibodies at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc/2 fragments after 30 min incubation.įabRICATOR is available in seven different product formats: This article received 11 testimonials and 88% of readers who voted found it helpful, earning it our reader-approved status. WikiHow marks an article as reader-approved once it receives enough positive feedback. There are 14 references cited in this article, which can be found at the bottom of the page. She studied at the Upright Citizens Brigade Theatre and at New York University (NYU) Tisch in the TV Writing Certificate Program. Kendall runs an IRL internet comedy show at Caveat called Extremely Online, and a comedy show for called Sugarp!ss at Easy Lover. She has also written and directed content for the Netflix is a Joke social channels and has written marketing scripts for Between Two Ferns: The Movie, Astronomy Club, Wine Country, Bash Brothers, Stand Up Specials and more. Her films have screened at Indie Short Fest, Brooklyn Comedy Collective, Channel 101 NY, and 8 Ball TV. Kendall specializes in directing, writing, and producing comedic short films. Kendall Payne is a Writer, Director, and Stand-up Comedian based in Brooklyn, New York. This article was co-authored by Kendall Payne.

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